my question is what will the auto do? If it gets pollinated what type of seed will it produce? Will they be auto's or does it reset them back to regs?
The ruderalis autoflowering trait for photo neutrality is homozygous recessive. This means a plant needs two recessive genes (one from each parent) to be an autoflower. If you cross an auto with a regular, then the resultant genotype would be heterozygous for photo neutrality, and the phenotype would be "photo-regular" for every offspring in the bunch. However, if you then cross those heterozygous offspring with each other (F1 generation), then 1/4 offspring would be homozygous recessive for photo neutrality (phenotypically autos), 1/2 would be heterozygous for photo-neutrality (phenotypically regular photo periods, but each carrying 1 recessive "auto" gene), and 1/4 would be homozygous dominant (phenotypically regular photo periods).
I can't do an html table here, so I will try a punnet square with text:
FOR F1 GENERATION:
CAPITAL P is dominant "Photo-regular"
lower-case p is recessive "Photo-Neutral" or Autoflowering. The plant needs 2 p's to be phenotypically an autoflower.
P p
-----------
P | PP |Pp |
-----------
p |Pp |pp |
-----------
In this crossing of the first filial generation, only 1/4 of the offspring get both lower-case red p's, so only those will be Autos. The rest, either all green capital P's or mix of green captial P's and red lower-case p's will be regular photos, but half of them will be carrying one recessive photo-neutral gene.
Note: I tried to color-code this to make it easier, but it looks like the forum's CSS won't allow it. Also, although I understand genetics and modes of inheritance, I am not a plant biologist and I'm not sure of the name or symbol used for this particular gene, but regardless, it is just a text model for a gene, and the principles are correct, so "P" works just fine. Lastly, plant genetics can be weird, and although this is accurate, lines need to be stabilized to solidify the traits.
Hope this helps.
To see a World in a Grain of Sand, And a Heaven in a Wild Flower,
Hold Infinity in the palm of your hand, And Eternity in an hour.
― William Blake
@im_sparky some solid info there buddy. Thanks for the time it took to put that together in an understandable context. I'm usually baffled by scientific explanations but that's pretty straightforward
"There I go.
E-type flow."
@improper_weed_viking Glad to help. It only took a few minutes — no sweat.
To see a World in a Grain of Sand, And a Heaven in a Wild Flower,
Hold Infinity in the palm of your hand, And Eternity in an hour.
― William Blake
Great effort by everybody involved in this thread just starting my seed making journey and this has encouraged me so much, I don't know why I didn't see this earlier but let's make seeds and over grow the world !!
More fun in the seed making run.
A reversed Pineapple Chunk is surrounded by 3 potential matches. One Pineapple Chunk (of course 😉), one Strawberry Cough, and one Nakatomi Plaza. By the looks of the pistils there has been some activity happening and more feminized seeds should be in the making.
Then there’s the mostly reversed White Widow entertaining a Strawberry Cough clone and a White Widow female. The treated females get purple pots signifying that they cannot be consumed! Got 4 other plants trying to go the reverse route while these get their pollination on. This reversed female is actually throwing enough pollen for me to see in the lights when I shake it 🎉. Should be more feminized seeds here soon too!
The third tent is getting hand pollinated with harvested Strawberry Cough feminized pollen. That tent has Acapulco Gold, Nakatomi Plaza, and Jack Herer Fast flowering so 🤞 fingers crossed that the pollen was good. We will know in a week or so.
Blue dream has been dragging behind all the others. She is just beginning to throw pistils and the reversed plant has been sprayed 3 times so far. Here’s hoping for success
Random acts of kindness make the world a better place
@monkeydo, you used STS on all these? Getting the most mileage out of the mix? lol I got mine but not ready to start yet, yours sound great hope they all work out. Should be some good seeds if they do, good combo's.
@woodi2 yes, this is my homemade STS and I’m still working on getting the concentration correct. The first attempt was too weak and 3 females didn’t reverse, 2 did. I’ve doubled the concentration now and am trying the stubborn ones again. So far it looks good but it’s still early days.
Random acts of kindness make the world a better place
@monkeydo, is there anyway to measure the strength of the solution, PPM or anything?
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